What is the DNase test primarily used to assess?

What is the DNase test primarily used to assess?

• A. Ability to ferment lactose
• B. Production of deoxyribonuclease
• C. Presence of oxidase enzyme
• D. Hydrolysis of starch

Answer: (B)

The DNase test is primarily used to assess the production of deoxyribonuclease, an enzyme that catalyzes the degradation of DNA. When a microorganism is capable of producing DNase, it will hydrolyze the DNA present in the medium, leading to a change in the agar’s appearance. Typically, a DNase test is performed on agar plates containing DNA, and a positive result is indicated by a clear zone around the colony where the DNA has been broken down. This ability to produce DNase can be an important differentiating factor for species, particularly in identifying Staphylococcus aureus, which is known to produce this enzyme. Other options relate to different biochemical tests that assess various metabolic capabilities of bacteria. The ability to ferment lactose pertains to lactose fermentation tests, the presence of oxidase enzyme relates to oxidase tests, and the hydrolysis of starch is evaluated through starch hydrolysis tests. Each of these tests targets different enzymatic activities or metabolic processes, making them specific to their respective assessments, unlike the DNase test which focuses solely on deoxyribonuclease production.

The DNase test is primarily used to assess the production of deoxyribonuclease, an enzyme that catalyzes the degradation of DNA. When a microorganism is capable of producing DNase, it will hydrolyze the DNA present in the medium, leading to a change in the agar’s appearance. Typically, a DNase test is performed on agar plates containing DNA, and a positive result is indicated by a clear zone around the colony where the DNA has been broken down. This ability to produce DNase can be an important differentiating factor for species, particularly in identifying Staphylococcus aureus, which is known to produce this enzyme.

Other options relate to different biochemical tests that assess various metabolic capabilities of bacteria. The ability to ferment lactose pertains to lactose fermentation tests, the presence of oxidase enzyme relates to oxidase tests, and the hydrolysis of starch is evaluated through starch hydrolysis tests. Each of these tests targets different enzymatic activities or metabolic processes, making them specific to their respective assessments, unlike the DNase test which focuses solely on deoxyribonuclease production.