Which methods are used for testing the production of beta-lactamase?

Which methods are used for testing the production of beta-lactamase?

• A. Fluorescent staining and acidometric method
• B. Chromogenic cephalosporin method, acidometric method, and idiometric method
• C. Microscopy and culture methods
• D. PCR and western blot

Answer: (B)

The production of beta-lactamase, which contributes to antibiotic resistance by breaking down beta-lactam antibiotics, is often tested using a variety of methods that are effective in detecting this enzymatic activity. The chromogenic cephalosporin method stands out as a primary approach since it involves the use of specific substrates that will change color in the presence of beta-lactamase activity. This allows for a straightforward and visually identifiable result indicating whether the organism is producing the enzyme. Additionally, the acidometric method measures changes in pH that occur as beta-lactam antibiotics undergo hydrolysis due to beta-lactamase activity. This is another reliable way to assess the enzyme's production. The idiometric method could also involve the use of indicators that respond to the presence of beta-lactamase, though it is less common compared to the first two methods mentioned. In summary, this combination of methods effectively aids in the detection and quantification of beta-lactamase production, making them essential tools in combating antibiotic resistance in clinical microbiology.

The production of beta-lactamase, which contributes to antibiotic resistance by breaking down beta-lactam antibiotics, is often tested using a variety of methods that are effective in detecting this enzymatic activity.

The chromogenic cephalosporin method stands out as a primary approach since it involves the use of specific substrates that will change color in the presence of beta-lactamase activity. This allows for a straightforward and visually identifiable result indicating whether the organism is producing the enzyme.

Additionally, the acidometric method measures changes in pH that occur as beta-lactam antibiotics undergo hydrolysis due to beta-lactamase activity. This is another reliable way to assess the enzyme's production.

The idiometric method could also involve the use of indicators that respond to the presence of beta-lactamase, though it is less common compared to the first two methods mentioned.

In summary, this combination of methods effectively aids in the detection and quantification of beta-lactamase production, making them essential tools in combating antibiotic resistance in clinical microbiology.