Which of the following is NOT a method for testing the production of beta-lactamase?

Which of the following is NOT a method for testing the production of beta-lactamase?

• A. Saccharogenic method
• B. Chromogenic cephalosporin method
• C. Acidometric method
• D. Idiometric method

Answer: (A)

The saccharogenic method is not utilized for testing the production of beta-lactamase because it primarily involves measuring the fermentation of carbohydrates to produce acid or gas, rather than evaluating the enzymatic action of beta-lactamase on antibiotics. Beta-lactamase production is typically assessed through methods that directly measure the breakdown of beta-lactam antibiotics. In contrast, the chromogenic cephalosporin method is specifically designed to detect beta-lactamase activity by using a substrate that, upon hydrolysis by the enzyme, produces a color change, indicating the presence of the enzyme. The acidometric method involves measuring pH changes as beta-lactamase hydrolyzes specific antibiotics, while the idiometric method also assesses enzyme activity through specific indicators related to the breakdown of beta-lactam antibiotics. Thus, the saccharogenic method does not pertain to beta-lactamase testing, making it the appropriate choice for the question regarding methods that do not assess beta-lactamase production.

The saccharogenic method is not utilized for testing the production of beta-lactamase because it primarily involves measuring the fermentation of carbohydrates to produce acid or gas, rather than evaluating the enzymatic action of beta-lactamase on antibiotics. Beta-lactamase production is typically assessed through methods that directly measure the breakdown of beta-lactam antibiotics.

In contrast, the chromogenic cephalosporin method is specifically designed to detect beta-lactamase activity by using a substrate that, upon hydrolysis by the enzyme, produces a color change, indicating the presence of the enzyme. The acidometric method involves measuring pH changes as beta-lactamase hydrolyzes specific antibiotics, while the idiometric method also assesses enzyme activity through specific indicators related to the breakdown of beta-lactam antibiotics.

Thus, the saccharogenic method does not pertain to beta-lactamase testing, making it the appropriate choice for the question regarding methods that do not assess beta-lactamase production.